Insulin replacement restores the vesicular secretory apparatus in the diabetic rat lacrimal gland

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Relationship between the golgi apparatus, gerl, and secretory granules in acinar cells of the rat exorbital lacrimal gland

The method of secretory granuleformation in the acinar cells of the rat exorbital lacrimal gland was studied by electron microscope morphological and cytochemical techniques. Immature secretory granules at the inner face of the Golgi apparatus were frequently attached to a narrow cisternal structure similar to GERL as described in neurons by Novikoff et al. (Novikoff, P. M., A. B. Novikoff, N. ...

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The lacrimal apparatus

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Age-dependent changes in rat lacrimal gland anti-oxidant and vesicular related protein expression profiles

PURPOSE Anti-oxidation and exocytosis are important for maintaining exocrine tissue homeostasis. During aging, functional and structural alterations occur in the lacrimal gland (LG), including oxidative damage to proteins, lipids, and DNA. The aims of the present study were to determine in the aging LG: a) the effects of aging on LG structure and secretory activity and b) changes in the express...

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Lacrimal gland primary acinar cell culture: the role of insulin.

PURPOSE The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. METHODS LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentratio...

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ژورنال

عنوان ژورنال: Arquivos Brasileiros de Oftalmologia

سال: 2015

ISSN: 0004-2749

DOI: 10.5935/0004-2749.20150041